منابع مشابه
Simple buffers for 3D STORM microscopy
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging: making dye molecules blink. This usually relies on the utilization of complex buffers, containing different chemicals and sensitive enzymatic systems, limitin...
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Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolutions down to a few tens of nanometers by exploiting the cycling of dyes between fluorescent and non-fluorescent states to obtain a sparse popula...
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Virtualization has recently become very popular in the area of system engineering and administration. This is primarily due to its benefits, such as: longer uptimes, better hardware utilization, and greater reliability. These benefits can reduce physical infrastructure, space, power consumption, and management costs. However, managing a virtualized environment to gain those benefits is difficul...
متن کاملComparing methods for 3D microscopy
In previous studies, we examined three-dimensiional images of a latex bead containing a surface layer of fluorescence whose thickness was determined by physical sectioning. Confocal images of the bead from six different microscopes all exhibited a significantly thicker fluorescent shell than actually present. In contrast, deconvolved wide-field images of the bead produced an image with the corr...
متن کاملTest Samples for Optimizing STORM Super-Resolution Microscopy
STORM is a recently developed super-resolution microscopy technique with up to 10 times better resolution than standard fluorescence microscopy techniques. However, as the image is acquired in a very different way than normal, by building up an image molecule-by-molecule, there are some significant challenges for users in trying to optimize their image acquisition. In order to aid this process ...
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ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2013
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.4.000885